· Functional test ex vivo

來自體內(nèi)的功能測試

· Evaluation of single cells to detect heterogenous populations

評價檢測單個細(xì)胞異質(zhì)種群

· Whole blood assay: No isolation procedures and optimal culture medium

全血檢測：沒有隔離程序和*培養(yǎng)基

· Physiological stimulans for phagocytes: Bacteria and fMLP

吞噬細(xì)胞生理興奮勁：細(xì)菌和fMLP

· Dose response: Low and high stimulant

沒有靜電工件比較乳膠珠子聚苯乙烯管內(nèi)

· Standardized test procedure

標(biāo)準(zhǔn)化測試程序

· Exclusion of aggregation artifacts by DNA staining

通過DNA染色排除聚合工件

· Compatible with whole blood of mice and rats

兼容的小鼠和大鼠全血

· Fast assay: Whole assay time is 1.5 hours

快速測定:整個試驗時間是1.5小時

100 analyses. Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Blood. Evaluation by flow cytometry.

100次分析。臨床診斷人體全血白細(xì)胞氧化破裂的定量檢測。通過流式細(xì)胞術(shù)進(jìn)行評估。

Clinical Diagnostic for the Quantitative Analysis of Leukocyte Oxidative Burst in Human Whole Bloods

SUMMARY and EXPLANATION

BURSTTEST (PHAGOBURST) allows the quantitative determination of leukocyte oxidative burst in heparinized whole blood. It contains unlabeled opsonized bacteria (E.coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP) as stimulants, Dihydrorhodamine (DHR) 123 as a fluorogenic substrate and necessary reagents. It determines the percentage of phagocytic cells which produce reactive oxidants (conversion of DHR 123 to R 123) and their enzymatic activity (amount of R 123 per cell).

The evaluation of these bioactivities should be performed by flow cytometry.

BURSTTEST(PHAGOBURST)允許定量測定白細(xì)胞氧化闖入肝素化全血。 它包含未標(biāo)記的促進(jìn)調(diào)理作用的細(xì)菌(大腸桿菌),佛波醇12十四烷酸乙酸13(PMA)和趨化作用的多肽n甲酰遇見亮氨酸板式換熱器(fMLP),二氫若丹明(DHR)123作為一個熒光襯底和必要的試劑。它決定了吞噬細(xì)胞產(chǎn)生活性氧化劑的比例(轉(zhuǎn)換DHR 123 到 R 1233)及其酶活性(每個細(xì)胞R 123的數(shù)量)。

這些生物活性的測定應(yīng)該有流式細(xì)胞術(shù)完成。