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信使RNA（mRNA）5 '帽狀結(jié)構(gòu)能夠防止mRNA在細(xì)胞質(zhì)中的降解，促進(jìn)核糖體增長(zhǎng)和蛋白的翻譯。Trilink的帽類似物能夠?qū)崿F(xiàn)共轉(zhuǎn)錄加帽，這樣就省去了加帽酶加帽操作。
CleanCap AG是TriLink公司用于5 '共轉(zhuǎn)錄加帽的產(chǎn)品，CleancapAG加帽可以形成Cap 1結(jié)構(gòu)。CleancapAG的加帽效率高達(dá)98%。請(qǐng)注意：TriLink建議盡可能使用CleanCap AG，模板應(yīng)該使用配套的AG啟動(dòng)子。Cleancap AG的加帽效率通常>90%。不像CleanCap AG，使用CleanCap GG(貨號(hào)cat#N-7133或cat#N-7433)可能導(dǎo)致類似的ARCA轉(zhuǎn)錄效率和產(chǎn)量下降的現(xiàn)象。
上海起發(fā)trilink現(xiàn)貨產(chǎn)品：
| 貨號(hào) | 品名 | 規(guī)格 | 品牌 |
| N-7003-10 | ARCA | 10umol | trilinkbiotech |
| L-7702-100 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 100ug | trilinkbiotech |
| N-2060-1 | 5-Hydroxymethyl-2-dCTP | 1umol | trilinkbiotech |
| n-1013-1 | N6-Methyladenosine-5-TP | 1umol | trilinkbiotech |
| N-4004-1 | 2,3-Dideoxythymidine-5-Triphosphate | 1umol | trilinkbiotech |
| N-7003-5 | ARCA | 5umol | trilinkbiotech |
| N-1087-1 | 5-Hydroxymethylcytidine-5-Triphosphate 5-Hydroxymethyl-CTP | 1umol | trilinkbiotech |
| L-7606-1000 | CleanCap Cas9 mRNA | 1mg | trilinkbiotech |
| L-7608-1000 | CleanCap beta gal mRNA | 1mg | trilinkbiotech |
| N-2064-1 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
| N-1084-1 | ON HOLD 5-Carboxy-Cytidine-5-Triphosphate 5-Carboxy-CTP 5-caCTP | 1umol | trilinkbiotech |
| N-1010-10 | 2-Fluoro-2-deoxyuridine-5-Triphosphate | 10umol | trilinkbiotech |
| N-1008-10 | 2-Fluoro-2-deoxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-1014-5 | 5-Methylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-1087-5 | 5-Hydroxymethylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-8006-1 | Cytidine-5-(1-Thiotriphosphate) | 1umol | trilinkbiotech |
| N-8008-1 | 1-Thio-Uridine-5-Triphosphate | 1umol | trilinkbiotech |
| L-7702-1000 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 1mg | trilinkbiotech |
| N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-5002-050 | 生物標(biāo)記dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
| N-1087-10 | 5-Hydroxymethylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-1512-25 | Guanosine-5-Triphosphate | 25umol | trilinkbiotech |
| N-1084-5 | 5-Carboxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-1085-1 | 5-Formylcytidine-5-Triphosphate 5-Formyl-CTP | 1umol | trilinkbiotech |
| L-7606-100-100 ug | CleanCap Cas9 mRNA | 100ug | trilinkbiotech |
| N-1066-1 | 8-Oxoguanosine-5-Triphosphate | 1umol | trilinkbiotech |
| N-1066-5 | 8-Oxoguanosine-5-Triphosphate | 5umol | trilinkbiotech |
| N-1065-1 | 5-Aminoallylcytidine-5-TP | 1umol | trilinkbiotech |
| N-2063-5 | 5-Carboxy-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| L-7608-100 | CleanCap beta gal mRNA | 100µg | trilinkbiotech |
| N-1085-10 | 5-Formylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-2020-1 | 2-Deoxyuridine-5-Triphosphate | 1umol | trilinkbiotech |
| N-1084-10 | 5-Carboxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-5002-050 | 生物標(biāo)記dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
| N-2063-5 | 5-Carboxy-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-1087-10 | 5-Hydroxymethylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-1084-10 | 5-Carboxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
| N-1065-1 | 5-Aminoallylcytidine-5-TP | 1umol | trilinkbiotech |
| N-1085-5 | 5-Formylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-1087-1 | 5-Hydroxymethylcytidine-5-Triphosphate 5-Hydroxymethyl-CTP | 1umol | trilinkbiotech |
| N-1084-5 | 5-Carboxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-4004-5 | 2,3-Dideoxythymidine-5-Triphosphate | 5umol | trilinkbiotech |
| L-7702-1000 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 1mg | trilinkbiotech |
| N-1010-1 | 2-Fluoro-2-deoxyuridine-5-TP | 1umol | trilinkbiotech |
| N-5002-050 | 生物標(biāo)記dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
| N-8008-1 | 1-Thio-Uridine-5-Triphosphate | 1umol | trilinkbiotech |
| N-8002-5 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-8001-5 | 1-Thio-2-Deoxyadenosine-5-TP | 5umol | trilinkbiotech |
| N-2064-10 | 5-Formyl-2-deoxycytidine-5-TP | 10umol | trilinkbiotech |
| N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| N-8002-1 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
| N-8001-1 | 1-Thio-2-Deoxyadenosine-5-Triphosphate | 1umol | trilinkbiotech |
| N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
| n-1013-1 | N6-Methyladenosine-5-TP | 1umol | trilinkbiotech |
| N-8002-1 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
| N-1008-1 | 2-Fluoro-2-deoxycytidine-5-TP | 1umol | trilinkbiotech |
| N-2020-5 | 2-Deoxyuridine-5-Triphosphate | 5umol | trilinkbiotech |
Cleancap AU是mRNA表達(dá)過(guò)程中用于共轉(zhuǎn)錄加帽的產(chǎn)品，能夠同步生產(chǎn)出具有天然結(jié)構(gòu)Cap 1的mRNA。CleanCapAU是專為自復(fù)制RNA(saRNA)設(shè)計(jì)的，該saRNA基于正義RNA病毒，如委內(nèi)瑞拉馬腦炎病毒(VEEV)、塞姆利基森林病毒(SFV)和辛德比斯病毒(SIN)的基因組。這些正義RNA病毒基因組起始是一個(gè)5'-AU…Cap 1 mRNA。與傳統(tǒng)的共轉(zhuǎn)錄轉(zhuǎn)錄加帽方法產(chǎn)生的Cap 0 mRNA相比，具有更高的體內(nèi)活性。CleanCap可以與TriLink的修飾的NTP產(chǎn)品一起使用。
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